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KMID : 0861020150300020031
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Korea Journal of Herbology
2015 Volume.30 No. 2 p.31 ~ p.36
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Antioxidative Activities and Inhibition of DNA Damage of Ethylacetate Fraction from Sophorae Flos and Sophorae fructus
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Jang Tae-Won
Kim Ye-Rang
Lee Sung-Hyeon
Kim Do-Wan
Park Jae-Ho
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Abstract
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Objectives : In this study, we demonstrated the antioxidant activities and the inhibitory effect on oxidative DNA damage of ethyl acetate fractions extracted from Sophorae Flos and Sophorae fructus.
Methods : Sophorae Flos and Sophorae fructus were extracted with methanol(MeOH) and divided to Petroleum ether, Ethyl acetate(EtOAC) and Water fraction. The antioxidant activities were conducted by the 1,1-diphenyl-2-picryl hydrazyl(DPPH) radical, 2, 2``-Azine-bis(3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt(ABTS) radical scavenging assay, Fe2+ chelating assay and Reducing power assay. The inhibitory effect of DNA damage were characterized on φX-174 RF I plasmid DNA cleavage assay. In addition, we analyzed the Total phenol contents and the Vitamin C contents of Sophorae Flos and Sophorae fructus.
Results : The results of DPPH were 92.71% and 94.72%, ABTS were 87.16% and 62.44%, and Fe2+ chelating were 95.81% and 85.11% at 200㎍/㎖of Sophorae Flos and Sophorae fructus respectively. The Sophorae Flos showed stronger effect than Sophorae fructus in Reducing Power assay. Total phenol content was 111.77 ㎎/g and 122.54 ㎎/g, and Vitamin C content was 2.59 ㎎/g and 3.03 ㎎/g. Also both Sophorae Flos and Sophorae fructus have inhibitory antioxidant effect on φX-174 RF I plasmid DNA cleavage assay.
Conclusions : Over all, this study suggests that Sophorae Flos and Sophorae fructus can be used as not only effective antioxidant but also natural medicine.
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KEYWORD
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Sophorae Flos, Sophorae fructus, DPPH, Reducing power, DNA damage, Fe2+ chelating
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